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image of Green Synthesis of Mentha spicata L.-derived Silver Nanoparticles: Evaluation of

Abstract

Introduction

L. (Lamiaceae) has been used in traditional medicine to cure indigestion, stomach aches, and diarrhea. This research aims to synthesize silver nanoparticles from aqueous extract of and to investigate its antioxidant, antibacterial, and anticancer activities.

Methods

The plant was extracted using maceration with water, and -silver nanoparticles (MAgNPs) were prepared using a 5 mM silver nitrate solution. The antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide scavenging assays. Antibacterial analysis was done through the agar disk diffusion method. The anticancer potential was evaluated using the cytotoxicity and MTT assays.

Results

The phytochemical screening revealed the presence of flavonoids and other phenolic compounds. The aqueous extract and silver nanoparticles scavenged the DPPH and hydrogen peroxide free radicals, with IC values of 1.556 mg/mL and 1.695 mg/mL, respectively. The bacterial strains were susceptible to the extract and silver nanoparticles, ith inhibitory zones ranging from 4 to 11 mm. The zeta analysis revealed a size of 70.08 nm and a potential of -13.17 mV. The XRD showed a crystalline structure of silver nanoparticles. The FTIR revealed a characteristic N-H stretching frequency. The extract and nanoparticles exhibited cytotoxic and anti-proliferative effects in vitro against MDA-MB-231 cancer cells, with a significant difference among means (p < ).

Discussion

There is an urgent need to screen and standardize medicinal plants with medicinal benefits and less toxicity, which also serve as chelating agents in drug delivery.

Conclusion

The ability of the extract to scavenge free radicals and inhibit bacterial growth may be due to its chemical constituents. MAgNPs may be a viable option for potential application and development in cancer therapy.

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2026-01-21
2026-03-03

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References

  1. Shoge M. Abdullateef A. Ozioko E.N. Muhammad N.A. Lawal H.A. Evaluation of heavy metals and antimicrobial efficacy of four hand sanitizers used during COVID-19 in AFIT, Kaduna. Int. J. Eng. Sci. 2021 1 1 24 27 10.9790/1813‑1001012427
    [Google Scholar]
  2. Zhang J. Jiang J. Yang Y. Wu T. Su X. Tao H. Growth and physiological responses of spearmint (Mentha spicata) cuttings to norfloxacin. Ecotoxicol. Environ. Saf. 2025 294 118058 10.1016/j.ecoenv.2025.118058 40112627
    [Google Scholar]
  3. Abdullateef A. Olajide R.A. Emmanuel E. Bolanle M.K. Umaru K. Changes in antioxidants in the brain of fluoride-treated rats. Asian J. Res. Med. Pharm. Sci. 2021 10 1 41 48 10.9734/AJRIMPS/2021/v10i130157
    [Google Scholar]
  4. Halilu E.M. Ngweh V.A. Airemwen C.O. Green synthesis of silver nanoparticles from Parinari curatellifolia methanol stem bark extract and evaluation of antioxidant and antimicrobial activities. Trop. J. Nat. Prod. Res. 2023 7 3 2498 2505 10.26538/tjnpr/v7i3.5
    [Google Scholar]
  5. Dayana S.M. Edwin H.V. Rajan M.R. Antony C.S. Ignacimuthu S. Phyto-synthesis of selenium nanoparticles using Mentha spicata (mint) extract and its larvicidal and antibacterial activities. J. Asia Pac. Entomol. 2025 28 1 102370 10.1016/j.aspen.2025.102370
    [Google Scholar]
  6. Soori H. Abdullateef A. Mashood L.O. Apietu K.A. A case series analysis of 1200 breast cancer patients with 12 years follow-up from a single hospital in Iran. Indian J. Surg 2025 2025 10.1007/s12262‑025‑04352‑6
    [Google Scholar]
  7. Haider L. Blank-Landeshammer B. Reiter N. Heckmann M. Iken M. Weghuber J. Röhrl C. Enhanced in-vitro bioavailability of curcumin, lutein and isoflavones through interaction with spearmint (Mentha spicata) via its bioactive component (R)-(-)-carvone. J. Nutr. Biochem. 2025 139 109868 10.1016/j.jnutbio.2025.109868 39984059
    [Google Scholar]
  8. Jamshidi-Kia F. Saeidi K. Lorigooini Z. Samani B.H. Efficacy of foliar application of Chlorella vulgaris extract on chemical composition and biological activities of the essential oil of spearmint (Mentha spicata L.). Heliyon 2024 10 23 e40531 10.1016/j.heliyon.2024.e40531 39660204
    [Google Scholar]
  9. Sivalingam A.M. Biosynthesis of ZnO nanocomposites from Mentha spicata applications of antioxidant, antimicrobial and genotoxicity advances in MCF-7 cell line. Sens. Biosensing Res. 2024 46 100707 10.1016/j.sbsr.2024.100707
    [Google Scholar]
  10. Esther A.S. Kumar J. Green and chemical syntheses of silver nanoparticles: Comparative and comprehensive study on characterization, therapeutic potential, and cytotoxicity. Eur. J. Med. Chem. Rep. 2024 11 100168 10.1016/j.ejmcr.2024.100168
    [Google Scholar]
  11. Yang J. Chen J. Hao Y. Liu Y. Identification of the DPPH radical scavenging reaction adducts of ferulic acid and sinapic acid and their structure-antioxidant activity relationship. Lebensm. Wiss. Technol. 2021 146 111411 10.1016/j.lwt.2021.111411
    [Google Scholar]
  12. Ayoubi R. Singh G. Pandey D.K. The effect of melatonin on essential oil production in Mentha Spicata. J. Nat. Sci. Rev. 2024 2 2 15 34 10.62810/jnsr.v2i2.43
    [Google Scholar]
  13. Vui N.V. Linh N.T. Quyen N.T.K. Nang K. Trinh L.T.T. The Interaction of Ocimum basilicum, Perilla frutescens and Mentha spicata essential oils with norfloxacin against antibiotic‐resistant Salmonella Spp. that cause disease in chickens. Vet. Med. Sci. 2025 11 2 e70316 10.1002/vms3.70316 40116564
    [Google Scholar]
  14. Luna R. P.F.; Munguia-Falcon, H.M.; Mogollon-Ortega, I.E.; Saavedra-Flores, E.E.; Romero-Ayala, C.P.; Siancas-Carrasco, M.F.; Márquez-Bautista, M.A.; Figueroa-Serrudo, C.E.; Montes-Yarasca, I.M.; Cántaro-Segura, H.B. Influence of Ascophyllum nodosum extract on rooting of Mentha spicata L. Agron. Costarric. 2024 48 2 149 156 10.15517/rac.v48i2.62549
    [Google Scholar]
  15. Dwi Octavia N. Pratiwi Puspitawati R. Bashri A. Characteristics of anatomical structure and essential oil glands of leaf peppermint (Mentha Piperita) and spearmint (Mentha Spicata). J. Wood Sci. 2023 2 9 1314 1329 10.58344/jws.v2i9.413
    [Google Scholar]
  16. Joshi S. Venkatesha K.T. Chandra Padalia R. Kumar D. First insights into the AMMI and GGE biplot-based multi-environment analysis for morpho-chemical traits in spearmint (Mentha spicata L.) half-sib genotypes. Ecol. Genet. Genom. 2024 32 100274 10.1016/j.egg.2024.100274
    [Google Scholar]
  17. Emre İ. Kurşat M. Yilmaz Ö. Erecevit P. Chemical compositions, radical scavenging capacities and antimicrobial activities in seeds of Satureja hortensis L. and Mentha spicata L. subsp. spicata from Turkey. Braz. J. Biol. 2021 81 1 144 153 10.1590/1519‑6984.224654 32401852
    [Google Scholar]
  18. Qaeed M.A. Examining the varied concentrations of Mentha spicata and Ocimum basilicum affect the synthesis of AgNPs that restrict the development of bacteria. Saudi J. Biol. Sci. 2024 31 1 103899 10.1016/j.sjbs.2023.103899 38125734
    [Google Scholar]
  19. Başyi̇ği̇t B. Çam M. Püskürtmeli Kurutucu ile Nane (Mentha piperita ve Mentha spicata) Esansiyel Yağı Mikroenkapsülasyonu. Harran Tarım ve Gıda Bilimleri Dergisi 2017 21 1 24 34 10.29050/harranziraat.303125
    [Google Scholar]
  20. Sainta Jostar T. Johnsy Arputhavalli G. Palaniyandy N. Jebasingh S. Alshahrani M.Y. Divya G.S. Muthu Vijayalakshmi P. Analyzing the synergistic effect of Mg on Mentha spicata L. mediated Mn2O3 nanoparticles for energy storage and bio-medical applications. Inorg. Chem. Commun. 2025 171 113539 10.1016/j.inoche.2024.113539
    [Google Scholar]
  21. Nandiyanto A.B.D. Oktiani R. Ragadhita R. How to read and interpret ftir spectroscope of organic material. Indones J. Sci. Technol 2019 4 1 97 118 10.17509/ijost.v4i1.15806
    [Google Scholar]
  22. D’Agostino A. Di Marco G. Canini A. Gismondi A. Gallic acid as a phytostimulant enhancing yield and quality of Mentha spicata L. under deficit- and well-watered conditions. Environ. Exp. Bot. 2024 219 105656 10.1016/j.envexpbot.2024.105656
    [Google Scholar]
  23. Akhayere E. Kavaz D. Vaseashta A. Efficacy studies of silica nanoparticles synthesized using agricultural waste for mitigating waterborne contaminants. Appl. Sci. 2022 12 18 9279 10.3390/app12189279
    [Google Scholar]
  24. Choudhary A. Salar R.K. Thakur R. Synthesis, characterization and insecticidal activity of Mentha spicata essential oil loaded polymeric nanoparticles. Biocatal. Agric. Biotechnol. 2024 55 102989 10.1016/j.bcab.2023.102989
    [Google Scholar]
  25. Bruna T. Maldonado-Bravo F. Jara P. Caro N. Silver nanoparticles and their antibacterial applications. Int. J. Mol. Sci. 2021 22 13 7202 10.3390/ijms22137202 34281254
    [Google Scholar]
  26. Araniti F. Prinsi B. Cocetta G. Negrini N. Nocito F.F. Espen L. Impact of cyclic-mild-drought stress on the metabolism of Mentha spicata L.: A strategy to improve quality traits. Ind. Crops Prod. 2024 210 118129 10.1016/j.indcrop.2024.118129
    [Google Scholar]
  27. Khalid Z. Javed A. Alyas T. Shaheen S. Nazish M. Khalid R. Obaid S.A. Ansari M.J. Kamal A. Biosynthesis, structural characterization of silver nanoparticles synthesized using an eco-friendly method with Mentha spicata L. extract and their antimicrobial activity and toxicological risk assessment. Results Chem. 2024 7 101487 10.1016/j.rechem.2024.101487
    [Google Scholar]
  28. Elbouzidi A. Haddou M. Baraich A. Taibi M. El Hachlafi N. Pareek A. Mesnard F. Addi M. Biochemical insights into specialized plant metabolites: Advancing cosmeceutical applications for skin benefits. J. Agric. Food Res. 2025 19 101651 10.1016/j.jafr.2025.101651
    [Google Scholar]
  29. Mani S.T. Jayakumar P. Pavithra M.E. Saranya K. Rathinavel T. Ammashi S. Green synthesis and characterization of silver nanoparticles from Eclipta alba and its activity against triple-negative breast cancer cell line (MDA-MB-231). Mol. Biotechnol. 2024 66 12 3597 3607 10.1007/s12033‑023‑00959‑w 37993758
    [Google Scholar]
  30. Bharati R. Fernández-Cusimamani E. Gupta A. Novy P. Moses O. Severová L. Svoboda R. Šrédl K. Oryzalin induces polyploids with superior morphology and increased levels of essential oil production in Mentha spicata L. Ind. Crops Prod. 2023 198 116683 10.1016/j.indcrop.2023.116683
    [Google Scholar]
  31. Xu L. Wang F. Liu Y. Wang M. Chen X. Wang E. Yan J. Beneficial elements silicon and calcium can be used as supplements to improve the yield and quality of the Mentha spicata L. essential oils. Ind. Crops Prod. 2024 214 118506 10.1016/j.indcrop.2024.118506
    [Google Scholar]
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Green Synthesis of Mentha spicata L.-derived Silver Nanoparticles: Evaluation of
Bentham Science Publishers ; https://doi.org/10.2174/0118715206387466250930053139
/content/journals/acamc/10.2174/0118715206387466250930053139
/content/journals/acamc/10.2174/0118715206387466250930053139
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  • Article Type:     Research Article
Keywords: Antibacterial ; phytochemicals ; anticancer ; Mentha spicata ; antioxidant ; breast cancer

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