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2000
Volume 25, Issue 10
  • ISSN: 1871-5206
  • E-ISSN: 1875-5992

Abstract

Introduction/Objective

Plants and their bioactive compounds play a crucial role in the pharmaceutical industry for treating cancer. To date, the cytotoxic and antiproliferative effects of methanol extract on human thyroid cancer cell lines have not been thoroughly explored. The present study aimed to assess the potential anti-cancer effects of HPME on human thyroid cancer and investigate its potential therapeutic benefits.

Methods

HPME was prepared using the maceration method, and its antioxidant activity was examined. Cytotoxicity studies were then carried out, followed by an investigation of the possible effects of HPME on metastasis and colony-forming capacities of human thyroid cancer cells. Afterward, qRT-PCR, western blotting, and apoptosis assays were performed.

Results

Cytotoxicity studies revealed notable cytotoxicity of HPME against the TT cell line. Moreover, HPME significantly curtailed metastasis and invasion of TT cells in an wound healing assay. Analyses of gene expressions demonstrated an elevation in caspase-12, caspase-3, and Bax, coupled with a reduction in BcL-2, APOE, and CLU expression. Following HPME treatment, there was an increase in the protein expression levels of Bax and Caspase-12, while a decrease in the BcL-2, APOE, and CLU protein expression. Furthermore, apoptotic studies indicated an increase in early apoptosis.

Conclusion

Overall results revealed that HPME demonstrates a notable antioxidant capacity in human thyroid cancer. It exerts an influence on crucial biological processes associated with cancer, indicating its potential to hinder the proliferation of human thyroid cancer cells by enhancing apoptosis through the upregulation of gene and protein expression, particularly involving caspases.

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2025-01-14
2025-10-15

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Investigation of the Anticarcinogenic Effects of Hypericum perforatum Extract on Human Thyroid Cancer
Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Cancer Agents) 25, 719 (2025); https://doi.org/10.2174/0118715206340411241120051020
/content/journals/acamc/10.2174/0118715206340411241120051020
/content/journals/acamc/10.2174/0118715206340411241120051020
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  • Article Type:     Research Article
Keyword(s): antioxidant capacity; apoptosis; cytotoxicity; Hypericum perforatum; protein expression; thyroid cancer

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