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2000
Volume 24, Issue 17
  • ISSN: 1871-5206
  • E-ISSN: 1875-5992

Abstract

Objective

() contains several bioactive compounds with anti-cancer activities. This study was performed to investigate the molecular effects of on HPV18-containing HeLa cells.

Methods

The methanol extract of whole plant was tested for cytotoxicity by MTT assay. A lower and higher dose (80 and 160 µg/mL) to IC were analyzed for colonization inhibition (Clonogenic assay), cell cycle arrest (FACS analysis), and induction of apoptosis (AO/EtBr staining fluorescent microscopy and FACS analysis) and DNA fragmentation (comet assay). The HPV 18 E6 gene expression in treated cells was analyzed using RT-PCR and qPCR.

Results

A significant dose-dependent anti-proliferative activity (IC - 108.25±2 µg/mL) and inhibition of colony formation cell line were observed using both treatments. Treatment with 80 µg/mL of extract was found to result in a higher percent of cell cycle arrest at G/G and GM phases with more early apoptosis, while 160 µg/mL resulted in more cell cycle arrest at SUBG and S phases with late apoptosis for control. The comet assay also demonstrated a highly significant increase in DNA fragmentation after treatment with 160 µg/mL of extract (tail moments-19.536 ± 17.8), while 80 µg/mL of extract treatment showed non-significant tail moment (8.152 ± 13.0) compared to control (8.038 ± 12.0). The RT-PCR and qPCR results showed a significant reduction in the expression of the HPV18 E6 gene in HeLa cells treated with 160 µg/mL of extract, while 80 µg/mL did not show a significant reduction.

Conclusion

The 160 µg/mL methanol extract of demonstrated highly significant anti-cancer molecular effects in HeLa cells.

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Molecular Actions of Enicostemma hyssopifolium Whole Plant Extract on HPV18-Infected Human Cervical Cancer (HeLa) Cells
Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Cancer Agents) 24, 1253 (2024); https://doi.org/10.2174/0118715206296375240703115848
/content/journals/acamc/10.2174/0118715206296375240703115848
/content/journals/acamc/10.2174/0118715206296375240703115848
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  • Article Type:     Research Article
Keyword(s): apoptosis-FACS; cell cycle arrest; clonogenic assay; comet assay; E. hyssopifolium; HeLa cell lines; HPV18 E6

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