Isolation and Use of Trabecular Meshwork Cells
- Authors: Najam A. Sharif1, Saima Chaudhry2
-
View Affiliations Hide Affiliations1 Vice President and Head of Research & Development Nanoscope Therapeutics Inc., 2777 N. Stemmons Fwy, Suite 1102, Dallas, TX 75207, USA 2 Univ N. Texas at Arlington, 701 S Nedderman Dr. Arlington, TX-76019, USA
- Source: Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma - Ocular Hypertension , pp 79-85
- Publication Date: August 2025
- Language: English
Isolation and Use of Trabecular Meshwork Cells, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/9789815324334/chapter-8-1.gif
As described elsewhere in the book, ocular hypertension (OHT) is a major risk factor for the development and progression of open-angle, closed-angle, and secondary forms of glaucoma. OHT or elevated intraocular pressure (IOP) is caused when the major aqueous humor (AQH)-drainage pathway, the trabecular meshwork (TM), and Schlemm's canal (SC) are slowly occluded by progressive deposition of extracellular matrix in and around the latter structures. In order to study the structure and function of the TM, most researchers isolate this tissue from different species, including from post-mortem human eyes, and either use the whole tissue (e.g., in contraction/relaxation assays) or isolate individual single cells and propagate them under tissue culture conditions. These cells can then be used for studying the morphological, biochemical, and pharmacological changes induced by various insults to simulate OHT/glaucomatous disease states or in response to compounds of interest utilizing various functional readouts.
-
From This Site
/content/books/9789815324334.chapter-8dcterms_subject,pub_keyword-contentType:Journal -contentType:Figure -contentType:Table -contentType:SupplementaryData105
