In vitro Propagation Protocol of Tylophora indica (Burm.f.)Merrill

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Tylophora indica (Burm.f.) Merrill is one of the most commonly used medicinal plants with bioactive alkaloid-rich secondary metabolites. This plant is used to treat asthma, dysentery, whooping cough, rheumatic pains, jaundice, and cancer. Rapid exploitation of this plant in natural habitats and poor regeneration methods, which are not in pace with those of destruction, make tissue culture methods a viable option to be used as a method of conservation. In the present chapter, tissue culture protocols have been reported till now as the best viable means in the rapid multiplication of T. indica. Sterilization protocols, callus induction and somatic embryogenesis methods, and direct and indirect organogenesis used by different researchers in mass propagation and acclimatization are given in detail. The present chapter gives an insight into the hormones needed and the response of the explants, which will be helpful for those who want to propagate this medicinal plant under in vitro conditions.