Qualitative and Quantitative Investigation of Bio Tissues using Microscopy and Data Mining
- Authors: Hafeez Ullah1, Munir Akhtar2, Muhammad Ramzan Khawar3
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View Affiliations Hide Affiliations1 Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia University ofBahawalpur, Bahawalpur, Pakistan 2 Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia University ofBahawalpur, Bahawalpur, Pakistan 3 Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia University ofBahawalpur, Bahawalpur, Pakistan
- Source: Recent Advances in Analytical Techniques: Volume 5 , pp 93-158
- Publication Date: January 2022
- Language: English
Qualitative and Quantitative Investigation of Bio Tissues using Microscopy and Data Mining, Page 1 of 1
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<div>The effects of glucose and salt on white blood cells, red blood cells, and</div><div>platelets (PLTs) in the blood of a leukemic patient by using a white light microscope</div><div>have been investigated for different concentrations (0 mM to 500 mM) of glucose and</div><div>salt. It has been revealed that the shape of erythrocytes, leukocytes, and platelets</div><div>changes and forms aggregates. Increasing the concentration of sodium chloride causes</div><div>an increase in the rouleaux formation and aggregation of platelets. The comparison of</div><div>CBC reports of these samples with and without analytes shows that total leukocyte</div><div>count (TLC) decreases gradually towards normal ranges of leukocytes, which is</div><div>favorable in the treatment of leukemia; at the same time, decreased level of hemoglobin</div><div>HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin</div><div>concentration (MCHC) and increased level of red blood cell (RBCs) causes a reduction</div><div>in the oxygen supply, which is in favor of cancer growth and anemia.<br><br></div><div>In the second set of work, a computer-aided system was planned for automatic</div><div>classification of ultrasound kidney diseases and ultrasound liver (i.e., cirrhosis). Two</div><div>types of images were considered normal and chronic. By using the data mining</div><div>technique, the statistical features were extracted to differentiate between normal and</div><div>abnormal ultrasonic kidney images. By using feature extraction software (FES), a set of</div><div>statistical features were extracted from the region of interest of each image at different</div><div>frame rates. The data sets which were obtained using FES at different frame rates were</div><div>then classified by using Weka. These extracted feature results were classified by using</div><div>Weka and a 96.5% correct classification rate was obtained. The difference between the</div><div>values of these features was useful to identify between normal and abnormal images.</div>
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