Circular Dichroism

- By Yusuf Tutar1
-
View Affiliations Hide Affiliations1 University of Health Sciences Turkey, Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, Turkey
- Source: Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications- Part II , pp 85-95
- Publication Date: August 2020
- Language: English
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Circular Dichroism (CD) is an absorption spectroscopy technique and measures left and right circularly polarized light difference of optical activity of asymmetric macromolecules. Unequal absorption of left and right circularly polarized light provides information about macromolecules depending on the spectral region. The far UV region provides data for secondary structure composition of proteins whereas near UV region provides data for tertiary structure of proteins. Furthermore, denaturation experiments provide stability parameters of proteins.
Hardbound ISBN:
9789811464843
Ebook ISBN:
9789811464867
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