Introduction To Physical Techniques for Determination of Structure of Biopolymers
- Authors: Nene Takio1, Meera Yadav2, SonamTashi Khom3
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View Affiliations Hide Affiliations1 Department of Chemistry, North Eastern Regional Institute of Science And Technology (NERIST), Nirjuli, Itanagar 791109, Arunachal Pradesh, India 2 Department of Chemistry, North Eastern Regional Institute of Science And Technology (NERIST), Nirjuli, Itanagar-791109, Arunachal Pradesh, India 3 Department of Chemistry, North Eastern Regional Institute of Science And Technology (NERIST), Nirjuli, Itanagar-791109, Arunachal Pradesh, India
- Source: Biochemistry: Fundamentals and Bioenergetics , pp 90-117
- Publication Date: October 2021
- Language: English
Introduction To Physical Techniques for Determination of Structure of Biopolymers, Page 1 of 1
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Biopolymers perform important cellular processes, which are isolated, purified and studied with the help of a wide range of biophysical, electrochemical and molecular biology techniques such as NMR, X-ray Crystallography, Electron Microscopy, and Electron Tomography. X-ray Crystallography helps us to establish 3D structures of small and large molecules at atomic resolution via the X-ray diffraction method, whereas NMR determines the content and purity of a sample. Electron microscopy obtains high-resolution images of biological and non-biological specimens and electron tomography provides three-dimensional density maps of the pleomorphic organism from macromolecular complexes to cells. All these techniques, discussed in this unit, provide us a greater insight to unravel the mystery of complex organisms and to understand the structure and functional properties of the biomolecules.
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