Production of Antibodies for Immunoanalytical Methods
- Authors: Ludek Eyer1, Milan Franek2
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View Affiliations Hide Affiliations1 Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic, Czech Republic 2 Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic, Czech Republic
- Source: Antibodies Applications and New Developments , pp 29-47
- Publication Date: May 2012
- Language: English
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Fundamental immunological principles and factors influencing production strategies for antibodies used as reagents in analytical and diagnostic methods are highlighted. A role of the peptideprotein design for an antibody formation, covering particularly epitope selection, peptide and protein chemistry, carrier-protein conjugation including multiple antigen protein approach are described. Special attention is paid to hapten design and production of antibodies against molecules having lowmolecular character. Critical stages in polyclonal and monoclonal antibody development as well as practical hints for their production are discussed.
Hardbound ISBN:
9781608055869
Ebook ISBN:
9781608052646
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